Blocking leukaemia inhibitory factor (LIF) has a negative effect on the implantation of mouse embryos. It has been determined that LIF regulates the expression of endometrial α_vβ₃ integrin, but its role in trophoblast cells remains unclear. The aim of this study was to evaluate the effect of 1000 units mL⁻¹ LIF on the expression of LIF receptor alpha (Lifr), integrin alpha V (Itgav), integrin beta 3 (Itgb3) and some apoptosis-related genes in blastocysts formed from 8-cell mouse embryos. Embryos obtained from superovulated NMRI mice were divided into four groups and cultured to the blastocyst stage. Groups 1 and 2 were simple embryo cultures in the absence and presence of LIF respectively; Groups 3 and 4 were embryo cocultures in the absence and presence of LIF respectively. The number of cells in blastocysts was counted and the expression of Itgav, Itgb3 and some apoptosis-related genes was determined using real-time polymerase chain reaction. The blastocyst rate, cell count, survival rate and expression of the Itgav and Itgb3 genes were significantly higher in the coculture groups and in the simple culture group with LIF than in the simple culture group without LIF. In conclusion, LIF improves the growth and cell count of blastocysts, increases the expression of the Itgav and Itgb3 genes in blastocysts and plays an important role in the success of implantation.

阻断白血病抑制因子（LIF）对小鼠胚胎的植入具有负面影响。已经确定的是LIF调节子宫内膜α的表达_v β ₃整合，但其在滋养层细胞的作用仍不清楚。这项研究的目的是评估1000单位毫升^-1 LIF对LIF受体α（Lifr），整合素αV（Itgav），整合素beta 3（Itgb3）表达的影响）和8细胞小鼠胚胎形成的胚泡中一些与凋亡相关的基因。从超排卵的NMRI小鼠获得的胚胎分为四组，并培养至胚泡期。第1组和第2组分别是在没有LIF和有LIF的情况下的简单胚胎培养。第3组和第4组分别是在不存在和存在LIF的情况下的胚胎共培养。实时聚合酶链反应计数囊胚中的细胞数，并确定Itgav，Itgb3和一些凋亡相关基因的表达。Itgav和Itgb3的胚泡率，细胞计数，存活率和表达共培养组和有LIF的简单培养组中的基因显着高于无LIF的简单培养组。总之，LIF改善了胚泡的生长和细胞计数，增加了胚泡中Itgav和Itgb3基因的表达，并且在成功植入中起着重要作用。